Project name: SH3_G131K

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA131K
Energy difference between WT (input) and mutated protein (by FoldX)	5.17339 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.2204
• Maximal score value: 1.7964
• Average score: -0.9784
• Total score value: -55.766

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9238
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5614
93	Y	A	-1.9343
94	E	A	-2.6487
95	A	A	-2.6957
96	R	A	-2.9865
97	T	A	-2.6618
98	E	A	-3.2053
99	D	A	-3.2204
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2487
103	F	A	0.0000
104	H	A	-2.7299
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5068
111	I	A	-0.1139
112	L	A	0.0787
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1730
120	W	A	-1.1546
121	E	A	-1.3436
122	A	A	0.0000
123	R	A	-1.7129
124	S	A	0.0000
125	L	A	0.1258
126	T	A	-0.4081
127	T	A	-0.6878
128	G	A	-1.2166
129	E	A	-1.9636
130	T	A	-1.7645
131	K	A	-1.9905	mutated: GA131K
132	Y	A	-1.1580
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964