Project name: SH3_L125Q

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA125Q
Energy difference between WT (input) and mutated protein (by FoldX)	1.62659 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7807
• Average score: -1.1109
• Total score value: -63.3186

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4929
86	L	A	0.4315
87	F	A	0.6335
88	V	A	0.1390
89	A	A	0.0000
90	L	A	-0.1132
91	Y	A	-0.5342
92	D	A	-2.5201
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.6773
105	K	A	-2.3033
106	G	A	-1.2416
107	E	A	-1.7246
108	K	A	-1.4474
109	F	A	0.0000
110	Q	A	-1.0696
111	I	A	-0.0918
112	L	A	0.1272
113	N	A	-0.8931
114	S	A	-1.1803
115	S	A	-1.5967
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0619
121	E	A	-1.1631
122	A	A	0.0000
123	R	A	-2.3472
124	S	A	0.0000
125	Q	A	-2.3023	mutated: LA125Q
126	T	A	-1.6168
127	T	A	-1.5021
128	G	A	-2.0714
129	E	A	-2.6188
130	T	A	-1.9623
131	G	A	-1.5051
132	Y	A	-0.8711
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1364
138	V	A	0.0000
139	A	A	0.4044
140	P	A	0.7367
141	V	A	1.7807