Project name: SH3_I111N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	IA111N
Energy difference between WT (input) and mutated protein (by FoldX)	4.20962 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7894
• Average score: -1.0358
• Total score value: -59.0392

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.3420
86	L	A	0.5561
87	F	A	0.7063
88	V	A	0.4149
89	A	A	0.0000
90	L	A	-0.1536
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4606
107	E	A	-1.3169
108	K	A	-0.7699
109	F	A	0.0000
110	Q	A	-1.0180
111	N	A	-1.0232	mutated: IA111N
112	L	A	-0.3041
113	N	A	-1.1387
114	S	A	-1.3550
115	S	A	-1.5781
116	E	A	-2.5508
117	G	A	-2.1263
118	D	A	-2.4378
119	W	A	-1.0967
120	W	A	-1.2613
121	E	A	-1.4002
122	A	A	0.0000
123	R	A	-1.9713
124	S	A	0.0000
125	L	A	0.0211
126	T	A	-0.4881
127	T	A	-0.8294
128	G	A	-1.3692
129	E	A	-2.2470
130	T	A	-1.8121
131	G	A	-1.5956
132	Y	A	-0.9692
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.0221
136	N	A	-1.1505
137	Y	A	-0.1267
138	V	A	0.0000
139	A	A	0.4087
140	P	A	0.7642
141	V	A	1.7894