Project name: SH3_V141R

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA141R
Energy difference between WT (input) and mutated protein (by FoldX)	1.01084 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 0.1984
• Average score: -1.14
• Total score value: -64.982

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	-0.3594
86	L	A	-0.4327
87	F	A	-0.3388
88	V	A	-0.5571
89	A	A	0.0000
90	L	A	-0.0871
91	Y	A	-0.5326
92	D	A	-2.5183
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.6477
105	K	A	-2.2684
106	G	A	-1.5401
107	E	A	-1.1064
108	K	A	-1.0512
109	F	A	0.0000
110	Q	A	-0.5108
111	I	A	-0.0693
112	L	A	0.1430
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1529
122	A	A	0.0000
123	R	A	-1.6770
124	S	A	0.0000
125	L	A	0.1984
126	T	A	-0.3538
127	T	A	-0.7714
128	G	A	-1.3219
129	E	A	-2.2214
130	T	A	-1.6797
131	G	A	-1.4991
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1501
137	Y	A	-0.1371
138	V	A	0.0000
139	A	A	-0.5210
140	P	A	-1.0353
141	R	A	-1.8342	mutated: VA141R