Project name: SH3_L112Q

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA112Q
Energy difference between WT (input) and mutated protein (by FoldX)	1.00717 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1023
• Maximal score value: 1.7964
• Average score: -1.1009
• Total score value: -62.749

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4682
86	L	A	0.7144
87	F	A	0.8561
88	V	A	0.3871
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5597
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6250
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1023
99	D	A	-3.0339
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2026
103	F	A	0.0000
104	H	A	-2.7280
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3252
108	K	A	-0.6925
109	F	A	0.0000
110	Q	A	-1.2128
111	I	A	-1.0676
112	Q	A	-1.8448	mutated: LA112Q
113	N	A	-1.9341
114	S	A	-1.6441
115	S	A	-1.8509
116	E	A	-2.5735
117	G	A	-2.1401
118	D	A	-2.4462
119	W	A	-1.0982
120	W	A	-1.4436
121	E	A	-1.7479
122	A	A	0.0000
123	R	A	-2.0295
124	S	A	0.0000
125	L	A	-0.0767
126	T	A	-0.5273
127	T	A	-0.8866
128	G	A	-1.5031
129	E	A	-2.2529
130	T	A	-1.9078
131	G	A	-1.6568
132	Y	A	-1.0282
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9074
136	N	A	-1.1500
137	Y	A	-0.1241
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7480
141	V	A	1.7964