Project name: SH3_I111R

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	IA111R
Energy difference between WT (input) and mutated protein (by FoldX)	3.88565 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7173
• Average score: -1.107
• Total score value: -63.1014

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.1830
86	L	A	0.3134
87	F	A	0.0000
88	V	A	0.3118
89	A	A	0.0000
90	L	A	-0.1785
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4857
107	E	A	-1.3488
108	K	A	-0.9044
109	F	A	0.0000
110	Q	A	-1.3930
111	R	A	-1.7077	mutated: IA111R
112	L	A	-0.6429
113	N	A	-1.3063
114	S	A	-1.4441
115	S	A	-1.5532
116	E	A	-2.5324
117	G	A	-2.1116
118	D	A	-2.4260
119	W	A	-1.0872
120	W	A	-1.3964
121	E	A	-1.5710
122	A	A	0.0000
123	R	A	-2.1353
124	S	A	0.0000
125	L	A	-0.0007
126	T	A	-0.4881
127	T	A	-0.8294
128	G	A	-1.3692
129	E	A	-2.2470
130	T	A	-1.8918
131	G	A	-1.6658
132	Y	A	-1.0393
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.1090
136	N	A	-1.1514
137	Y	A	-0.1509
138	V	A	0.0000
139	A	A	0.3367
140	P	A	0.6730
141	V	A	1.7173