Project name: SH3_G128V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA128V
Energy difference between WT (input) and mutated protein (by FoldX)	5.3258 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -0.8244
• Total score value: -46.9896

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5159
86	L	A	0.8071
87	F	A	0.9191
88	V	A	0.4273
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3111
108	K	A	-0.6219
109	F	A	0.0000
110	Q	A	-0.2269
111	I	A	-0.0090
112	L	A	0.1880
113	N	A	-0.8848
114	S	A	-1.1776
115	S	A	-1.5953
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0340
121	E	A	-1.1114
122	A	A	0.0000
123	R	A	-0.8809
124	S	A	0.0000
125	L	A	0.7174
126	T	A	0.1107
127	T	A	0.2442
128	V	A	0.7839	mutated: GA128V
129	E	A	-1.1630
130	T	A	-1.0776
131	G	A	-1.2420
132	Y	A	-0.8397
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7749
141	V	A	1.7964