Project name: SH3_V141Y

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA141Y
Energy difference between WT (input) and mutated protein (by FoldX)	1.10563 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.3794
• Average score: -0.9565
• Total score value: -54.5186

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.3992
86	L	A	0.7290
87	F	A	0.8511
88	V	A	0.3905
89	A	A	0.0000
90	L	A	-0.1337
91	Y	A	-0.5878
92	D	A	-2.5604
93	Y	A	-1.9318
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.6884
105	K	A	-2.3666
106	G	A	-1.4263
107	E	A	-1.1188
108	K	A	-0.3212
109	F	A	0.0000
110	Q	A	-0.4386
111	I	A	-0.0230
112	L	A	0.1378
113	N	A	-0.8903
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0578
121	E	A	-1.1558
122	A	A	0.0000
123	R	A	-1.6408
124	S	A	0.0000
125	L	A	0.2568
126	T	A	-0.3418
127	T	A	-0.7552
128	G	A	-1.3357
129	E	A	-2.2275
130	T	A	-1.6864
131	G	A	-1.4991
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1508
137	Y	A	-0.1398
138	V	A	0.0000
139	A	A	0.3144
140	P	A	0.5746
141	Y	A	1.3794	mutated: VA141Y