Project name: SH3_G131N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA131N
Energy difference between WT (input) and mutated protein (by FoldX)	6.31796 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1518
• Maximal score value: 1.7964
• Average score: -0.9775
• Total score value: -55.7174

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5137
86	L	A	0.7906
87	F	A	0.9204
88	V	A	0.4309
89	A	A	0.0000
90	L	A	-0.1503
91	Y	A	-0.5741
92	D	A	-2.5589
93	Y	A	-1.9312
94	E	A	-2.6448
95	A	A	-2.6576
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.1518
99	D	A	-3.1291
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2439
103	F	A	0.0000
104	H	A	-2.7262
105	K	A	-2.4041
106	G	A	-1.4553
107	E	A	-1.3021
108	K	A	-0.6382
109	F	A	0.0000
110	Q	A	-0.5117
111	I	A	-0.0832
112	L	A	0.1191
113	N	A	-0.8912
114	S	A	-1.1809
115	S	A	-1.5969
116	E	A	-2.5617
117	G	A	-2.1354
118	D	A	-2.4461
119	W	A	-1.1402
120	W	A	-1.1068
121	E	A	-1.2498
122	A	A	0.0000
123	R	A	-1.7410
124	S	A	0.0000
125	L	A	0.0704
126	T	A	-0.4640
127	T	A	-0.8074
128	G	A	-1.3634
129	E	A	-2.2806
130	T	A	-1.8009
131	N	A	-1.7708	mutated: GA131N
132	Y	A	-1.0062
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9224
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7739
141	V	A	1.7964