Project name: SH3_L90P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA90P
Energy difference between WT (input) and mutated protein (by FoldX)	7.04986 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8119
• Average score: -0.991
• Total score value: -56.4856

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5232
86	L	A	0.8046
87	F	A	0.8874
88	V	A	0.3497
89	A	A	0.0000
90	P	A	-0.6563	mutated: LA90P
91	Y	A	-0.8194
92	D	A	-2.6663
93	Y	A	-1.9952
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.8024
105	K	A	-2.5756
106	G	A	-1.5741
107	E	A	-1.3721
108	K	A	-0.6794
109	F	A	0.0000
110	Q	A	-0.5039
111	I	A	-0.0573
112	L	A	0.1429
113	N	A	-0.8883
114	S	A	-1.1799
115	S	A	-1.5966
116	E	A	-2.5603
117	G	A	-2.1347
118	D	A	-2.4456
119	W	A	-1.1055
120	W	A	-1.0569
121	E	A	-1.1536
122	A	A	0.0000
123	R	A	-1.7161
124	S	A	0.0000
125	L	A	0.0630
126	T	A	-0.4739
127	T	A	-0.8211
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.4995
132	Y	A	-0.8681
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9202
136	N	A	-1.2233
137	Y	A	-0.2810
138	V	A	0.0000
139	A	A	0.3250
140	P	A	0.7925
141	V	A	1.8119