Project name: SH3_R96D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	RA96D
Energy difference between WT (input) and mutated protein (by FoldX)	1.61569 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1531
• Maximal score value: 1.7964
• Average score: -0.9941
• Total score value: -56.6609

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9235
88	V	A	0.4306
89	A	A	0.0000
90	L	A	-0.1463
91	Y	A	-0.5642
92	D	A	-2.5391
93	Y	A	-1.9048
94	E	A	-2.6407
95	A	A	-2.6570
96	D	A	-2.9748	mutated: RA96D
97	T	A	-2.6927
98	E	A	-3.1531
99	D	A	-3.1367
100	D	A	-2.2289
101	L	A	0.0000
102	S	A	-2.2299
103	F	A	0.0000
104	H	A	-2.7180
105	K	A	-2.3993
106	G	A	-1.4592
107	E	A	-1.3102
108	K	A	-0.6438
109	F	A	0.0000
110	Q	A	-0.5090
111	I	A	-0.0566
112	L	A	0.1431
113	N	A	-0.8643
114	S	A	-1.1445
115	S	A	-1.5755
116	E	A	-2.5290
117	G	A	-2.0881
118	D	A	-2.3642
119	W	A	-0.9664
120	W	A	-0.9967
121	E	A	-1.1438
122	A	A	0.0000
123	R	A	-1.7154
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2400
130	T	A	-1.7227
131	G	A	-1.5517
132	Y	A	-0.9059
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8666
136	N	A	-1.1252
137	Y	A	-0.0993
138	V	A	0.0000
139	A	A	0.4159
140	P	A	0.7757
141	V	A	1.7964