Project name: SH3_Y132P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA132P
Energy difference between WT (input) and mutated protein (by FoldX)	2.66532 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.165
• Maximal score value: 1.7964
• Average score: -0.9926
• Total score value: -56.5797

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5183
86	L	A	0.7945
87	F	A	0.9246
88	V	A	0.4320
89	A	A	0.0000
90	L	A	-0.1508
91	Y	A	-0.5745
92	D	A	-2.5593
93	Y	A	-1.9310
94	E	A	-2.6446
95	A	A	-2.6533
96	R	A	-2.9848
97	T	A	-2.6606
98	E	A	-3.1257
99	D	A	-3.1650
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2312
103	F	A	0.0000
104	H	A	-2.7273
105	K	A	-2.4052
106	G	A	-1.4570
107	E	A	-1.3055
108	K	A	-0.6409
109	F	A	0.0000
110	Q	A	-0.5756
111	I	A	-0.2111
112	L	A	-0.0358
113	N	A	-1.0982
114	S	A	-1.3302
115	S	A	-1.6633
116	E	A	-2.6482
117	G	A	-2.2231
118	D	A	-2.5521
119	W	A	-1.3128
120	W	A	-1.4083
121	E	A	-1.8033
122	A	A	0.0000
123	R	A	-1.8516
124	S	A	0.0000
125	L	A	0.0596
126	T	A	-0.4724
127	T	A	-0.8204
128	G	A	-1.3520
129	E	A	-2.2284
130	T	A	-1.8460
131	G	A	0.0000
132	P	A	-1.3341	mutated: YA132P
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.0341
136	N	A	-1.1521
137	Y	A	-0.1259
138	V	A	0.0000
139	A	A	0.4149
140	P	A	0.7757
141	V	A	1.7964