Project name: SH3_Y93P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA93P
Energy difference between WT (input) and mutated protein (by FoldX)	15.7326 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1595
• Maximal score value: 1.7964
• Average score: -1.0368
• Total score value: -59.0952

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9282
88	V	A	0.4358
89	A	A	0.0000
90	L	A	-0.1708
91	Y	A	-0.6178
92	D	A	-2.6509
93	P	A	-2.1443	mutated: YA93P
94	E	A	-2.7683
95	A	A	-2.7410
96	R	A	-3.0523
97	T	A	-2.7139
98	E	A	-3.1595
99	D	A	-3.1380
100	D	A	-2.2837
101	L	A	0.0000
102	S	A	-2.3136
103	F	A	0.0000
104	H	A	-2.7803
105	K	A	-2.4377
106	G	A	-1.4623
107	E	A	-1.3120
108	K	A	-0.6450
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0573
112	L	A	0.1425
113	N	A	-0.8932
114	S	A	-1.1870
115	S	A	-1.6009
116	E	A	-2.5668
117	G	A	-2.1475
118	D	A	-2.4673
119	W	A	-1.1675
120	W	A	-1.1018
121	E	A	-1.1930
122	A	A	0.0000
123	R	A	-1.7161
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.7211
131	G	A	-1.5713
132	Y	A	-0.9683
133	I	A	0.0000
134	P	A	-0.6313
135	S	A	-0.9263
136	N	A	-1.1390
137	Y	A	-0.1194
138	V	A	0.0000
139	A	A	0.4245
140	P	A	0.7757
141	V	A	1.7964