Project name: SH3_F87Q

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	FA87Q
Energy difference between WT (input) and mutated protein (by FoldX)	4.14982 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.712
• Average score: -1.0002
• Total score value: -57.01

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4510
86	L	A	0.6238
87	Q	A	0.0000	mutated: FA87Q
88	V	A	0.2824
89	A	A	0.0000
90	L	A	-0.1785
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4857
107	E	A	-1.3903
108	K	A	-0.8025
109	F	A	0.0000
110	Q	A	-0.6325
111	I	A	-0.0381
112	L	A	0.2433
113	N	A	-0.8261
114	S	A	-1.1352
115	S	A	-1.5794
116	E	A	-2.5527
117	G	A	-2.1265
118	D	A	-2.4345
119	W	A	-1.0851
120	W	A	-0.9998
121	E	A	-1.1060
122	A	A	0.0000
123	R	A	-1.7841
124	S	A	0.0000
125	L	A	-0.2128
126	T	A	-0.5900
127	T	A	-0.8907
128	G	A	-1.4402
129	E	A	-2.2846
130	T	A	-1.7180
131	G	A	-1.4841
132	Y	A	-0.8439
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9335
136	N	A	-1.1466
137	Y	A	-0.1509
138	V	A	0.0000
139	A	A	0.3342
140	P	A	0.6651
141	V	A	1.7120