Project name: SH3_N113P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	NA113P
Energy difference between WT (input) and mutated protein (by FoldX)	4.07388 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -0.9323
• Total score value: -53.1419

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5166
86	L	A	0.7913
87	F	A	0.9229
88	V	A	0.4309
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6258
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0345
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6443
109	F	A	0.0000
110	Q	A	-0.4850
111	I	A	0.0214
112	L	A	0.2538
113	P	A	-0.5189	mutated: NA113P
114	S	A	-0.9961
115	S	A	-1.4829
116	E	A	-2.4576
117	G	A	-2.0724
118	D	A	-2.3933
119	W	A	-1.0268
120	W	A	-0.9133
121	E	A	-0.9697
122	A	A	0.0000
123	R	A	-1.7278
124	S	A	0.0000
125	L	A	0.0569
126	T	A	-0.4739
127	T	A	-0.8217
128	G	A	-1.3594
129	E	A	-2.2419
130	T	A	-1.6924
131	G	A	-1.4866
132	Y	A	-0.7970
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9106
136	N	A	-1.1500
137	Y	A	-0.1241
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7753
141	V	A	1.7964