Project name: SH3_W120T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA120T
Energy difference between WT (input) and mutated protein (by FoldX)	3.08187 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8562
• Average score: -0.9442
• Total score value: -53.8196

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5708
86	L	A	0.8935
87	F	A	1.1067
88	V	A	0.5168
89	A	A	0.0000
90	L	A	-0.1305
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4373
107	E	A	-1.2814
108	K	A	-0.5806
109	F	A	0.0000
110	Q	A	-0.4496
111	I	A	-0.0116
112	L	A	0.1261
113	N	A	-0.9663
114	S	A	-1.3239
115	S	A	-1.6822
116	E	A	-2.6467
117	G	A	-2.2541
118	D	A	-2.5478
119	W	A	-1.2312
120	T	A	0.0000	mutated: WA120T
121	E	A	-1.2461
122	A	A	0.0000
123	R	A	-1.7168
124	S	A	0.0000
125	L	A	0.0772
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6846
131	G	A	-1.5209
132	Y	A	-0.9291
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9903
136	N	A	-1.1833
137	Y	A	-0.1140
138	V	A	0.0000
139	A	A	0.4706
140	P	A	0.8513
141	V	A	1.8562