Project name: SH3_K108C

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	KA108C
Energy difference between WT (input) and mutated protein (by FoldX)	1.31699 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.976
• Average score: -0.8726
• Total score value: -49.7396

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5540
86	L	A	1.1104
87	F	A	1.2672
88	V	A	0.8672
89	A	A	0.0000
90	L	A	-0.0520
91	Y	A	-0.5745
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.6131
105	K	A	-2.2747
106	G	A	-1.2151
107	E	A	-0.8329
108	C	A	0.3466	mutated: KA108C
109	F	A	0.0000
110	Q	A	-0.2636
111	I	A	0.0615
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.5464
124	S	A	0.0000
125	L	A	0.4569
126	T	A	-0.2233
127	T	A	-0.6999
128	G	A	-1.3315
129	E	A	-2.2266
130	T	A	-1.6834
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1240
138	V	A	0.0000
139	A	A	0.5374
140	P	A	0.9127
141	V	A	1.9760