Project name: SH3_G117N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA117N
Energy difference between WT (input) and mutated protein (by FoldX)	3.59481 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -1.0184
• Total score value: -58.0497

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9234
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5087
111	I	A	-0.0563
112	L	A	0.1437
113	N	A	-1.0186
114	S	A	-1.4284
115	S	A	-1.9045
116	E	A	-3.0338
117	N	A	-3.0947	mutated: GA117N
118	D	A	-2.9181
119	W	A	-1.3955
120	W	A	-1.2621
121	E	A	-1.2529
122	A	A	0.0000
123	R	A	-1.7151
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.5001
132	Y	A	-0.8703
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.0534
136	N	A	-1.1533
137	Y	A	-0.1266
138	V	A	0.0000
139	A	A	0.4153
140	P	A	0.7757
141	V	A	1.7964