Project name: SH3_W120Q

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA120Q
Energy difference between WT (input) and mutated protein (by FoldX)	2.55759 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8283
• Average score: -0.9545
• Total score value: -54.4039

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5416
86	L	A	0.8412
87	F	A	1.0164
88	V	A	0.4769
89	A	A	0.0000
90	L	A	-0.1401
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4470
107	E	A	-1.2937
108	K	A	-0.6122
109	F	A	0.0000
110	Q	A	-0.4976
111	I	A	-0.0831
112	L	A	0.0981
113	N	A	-0.9826
114	S	A	-1.3344
115	S	A	-1.6822
116	E	A	-2.6467
117	G	A	-2.2524
118	D	A	-2.5438
119	W	A	-1.2272
120	Q	A	0.0000	mutated: WA120Q
121	E	A	-1.2597
122	A	A	0.0000
123	R	A	-1.7307
124	S	A	0.0000
125	L	A	0.0688
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6913
131	G	A	-1.5266
132	Y	A	-0.9337
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9939
136	N	A	-1.1773
137	Y	A	-0.1193
138	V	A	0.0000
139	A	A	0.4448
140	P	A	0.8160
141	V	A	1.8283