Project name: SH3_S102C

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SA102C
Energy difference between WT (input) and mutated protein (by FoldX)	1.08703 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0379
• Maximal score value: 1.7964
• Average score: -0.9269
• Total score value: -52.8315

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9238
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1527
91	Y	A	-0.5780
92	D	A	-2.4666
93	Y	A	-1.7652
94	E	A	-2.4253
95	A	A	-2.4094
96	R	A	-2.9008
97	T	A	-2.5964
98	E	A	-3.0379
99	D	A	-2.9849
100	D	A	0.0000
101	L	A	0.0000
102	C	A	-1.6614	mutated: SA102C
103	F	A	0.0000
104	H	A	-2.6208
105	K	A	-2.4081
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.1750
130	T	A	-1.6359
131	G	A	-1.4210
132	Y	A	-0.7961
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1265
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964