Project name: SH3_S135G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SA135G
Energy difference between WT (input) and mutated protein (by FoldX)	-0.265198 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.806
• Average score: -0.9638
• Total score value: -54.9343

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5257
86	L	A	0.8091
87	F	A	0.9512
88	V	A	0.4453
89	A	A	0.0000
90	L	A	-0.1472
91	Y	A	-0.5743
92	D	A	-2.5596
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4057
106	G	A	-1.4548
107	E	A	-1.3036
108	K	A	-0.6328
109	F	A	0.0000
110	Q	A	-0.4984
111	I	A	-0.0596
112	L	A	0.1317
113	N	A	-0.9187
114	S	A	-1.2471
115	S	A	-1.6290
116	E	A	-2.5817
117	G	A	-2.1567
118	D	A	-2.4688
119	W	A	-1.1285
120	W	A	-1.0949
121	E	A	-1.1728
122	A	A	0.0000
123	R	A	-1.7142
124	S	A	0.0000
125	L	A	0.0621
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6915
131	G	A	-1.5001
132	Y	A	-0.8794
133	I	A	0.0000
134	P	A	0.0000
135	G	A	-0.9497	mutated: SA135G
136	N	A	-1.1608
137	Y	A	-0.1260
138	V	A	0.0000
139	A	A	0.4223
140	P	A	0.7878
141	V	A	1.8060