Project name: SH3_S102N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	SA102N
Energy difference between WT (input) and mutated protein (by FoldX)	1.06451 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.3797
• Maximal score value: 1.7964
• Average score: -1.0312
• Total score value: -58.7765

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9242
88	V	A	0.4320
89	A	A	0.0000
90	L	A	-0.1508
91	Y	A	-0.5745
92	D	A	-2.7721
93	Y	A	-2.3134
94	E	A	-3.1266
95	A	A	-3.0899
96	R	A	-3.1656
97	T	A	-2.7969
98	E	A	-3.2409
99	D	A	-3.1661
100	D	A	0.0000
101	L	A	0.0000
102	N	A	-3.3797	mutated: SA102N
103	F	A	0.0000
104	H	A	-2.9649
105	K	A	-2.4052
106	G	A	-1.4570
107	E	A	-1.3055
108	K	A	-0.6415
109	F	A	0.0000
110	Q	A	-0.5076
111	I	A	-0.0553
112	L	A	0.1446
113	N	A	-0.8883
114	S	A	-1.1799
115	S	A	-1.5966
116	E	A	-2.5603
117	G	A	-2.1347
118	D	A	-2.4456
119	W	A	-1.1053
120	W	A	-1.0569
121	E	A	-1.1507
122	A	A	0.0000
123	R	A	-1.7098
124	S	A	0.0000
125	L	A	0.0610
126	T	A	-0.4724
127	T	A	-0.8187
128	G	A	-1.3539
129	E	A	-2.3736
130	T	A	-1.8019
131	G	A	-1.6603
132	Y	A	-1.0175
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9214
136	N	A	-1.1508
137	Y	A	-0.1246
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964