Project name: SH3_G128W

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA128W
Energy difference between WT (input) and mutated protein (by FoldX)	4.06503 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.795
• Average score: -0.8761
• Total score value: -49.9357

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5315
86	L	A	0.7849
87	F	A	0.8876
88	V	A	0.3842
89	A	A	0.0000
90	L	A	-0.1516
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7287
105	K	A	-2.4065
106	G	A	-1.4592
107	E	A	-1.4101
108	K	A	-0.7445
109	F	A	0.0000
110	Q	A	-0.2794
111	I	A	0.0501
112	L	A	0.2173
113	N	A	-0.8813
114	S	A	-1.1803
115	S	A	-1.5967
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0170
121	E	A	-1.0827
122	A	A	0.0000
123	R	A	-1.0506
124	S	A	0.0000
125	L	A	0.1128
126	T	A	-0.2636
127	T	A	-0.1560
128	W	A	0.0999	mutated: GA128W
129	E	A	-1.4878
130	T	A	-1.2226
131	G	A	-1.2791
132	Y	A	-0.8265
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4150
140	P	A	0.7820
141	V	A	1.7950