Project name: SH3_D92V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	DA92V
Energy difference between WT (input) and mutated protein (by FoldX)	1.58638 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1026
• Maximal score value: 1.7964
• Average score: -0.7123
• Total score value: -40.6024

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9239
88	V	A	0.4314
89	A	A	0.0000
90	L	A	0.5750
91	Y	A	1.1566
92	V	A	0.9955	mutated: DA92V
93	Y	A	-0.2005
94	E	A	-1.8438
95	A	A	-2.2643
96	R	A	-2.9864
97	T	A	-2.6606
98	E	A	-3.1026
99	D	A	-3.0432
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-1.5649
103	F	A	0.0000
104	H	A	-1.1206
105	K	A	-1.0181
106	G	A	-0.9135
107	E	A	-0.8211
108	K	A	-0.6418
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0596
126	T	A	-0.4724
127	T	A	-0.8204
128	G	A	-1.3572
129	E	A	-2.2400
130	T	A	-1.6926
131	G	A	-1.4990
132	Y	A	-0.8672
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1508
137	Y	A	0.4111
138	V	A	0.0000
139	A	A	0.4153
140	P	A	0.7757
141	V	A	1.7964