Project name: SH3_D92F

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	DA92F
Energy difference between WT (input) and mutated protein (by FoldX)	1.27071 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1012
• Maximal score value: 1.7964
• Average score: -0.7138
• Total score value: -40.6887

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9248
88	V	A	0.4335
89	A	A	0.0000
90	L	A	0.5705
91	Y	A	1.1406
92	F	A	0.9625	mutated: DA92F
93	Y	A	-0.2194
94	E	A	-1.8498
95	A	A	-2.2648
96	R	A	-2.9854
97	T	A	-2.6592
98	E	A	-3.1012
99	D	A	-3.0420
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-1.5610
103	F	A	0.0000
104	H	A	-1.1330
105	K	A	-1.0299
106	G	A	-0.9157
107	E	A	-0.8207
108	K	A	-0.6393
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7155
124	S	A	0.0000
125	L	A	0.0606
126	T	A	-0.4713
127	T	A	-0.8199
128	G	A	-1.3572
129	E	A	-2.2385
130	T	A	-1.6913
131	G	A	-1.4973
132	Y	A	-0.8656
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	0.4057
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964