Project name: SH3_G131V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA131V
Energy difference between WT (input) and mutated protein (by FoldX)	8.4242 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0581
• Maximal score value: 1.7964
• Average score: -0.9389
• Total score value: -53.5168

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9248
88	V	A	0.4335
89	A	A	0.0000
90	L	A	-0.1501
91	Y	A	-0.5739
92	D	A	-2.5587
93	Y	A	-1.9310
94	E	A	-2.6446
95	A	A	-2.5948
96	R	A	-2.9850
97	T	A	-2.6607
98	E	A	-3.0581
99	D	A	-2.9609
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1648
103	F	A	0.0000
104	H	A	-2.7258
105	K	A	-2.4038
106	G	A	-1.4548
107	E	A	-1.3010
108	K	A	-0.6393
109	F	A	0.0000
110	Q	A	-0.5068
111	I	A	-0.0276
112	L	A	0.1755
113	N	A	-0.8849
114	S	A	-1.1771
115	S	A	-1.5966
116	E	A	-2.5603
117	G	A	-2.1347
118	D	A	-2.4426
119	W	A	-1.0687
120	W	A	-1.0077
121	E	A	-1.0589
122	A	A	0.0000
123	R	A	-1.6487
124	S	A	0.0000
125	L	A	0.0627
126	T	A	-0.4713
127	T	A	-0.8173
128	G	A	-1.3278
129	E	A	-2.1757
130	T	A	-1.5504
131	V	A	-1.2363	mutated: GA131V
132	Y	A	-0.7179
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9177
136	N	A	-1.1508
137	Y	A	-0.1246
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964