Project name: rbd1 [mutate: LC455R, FC486A, NC487K, EC484R, YC489V, FC490K]

Chain sequence(s)	C: TNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGP
Distance of aggregation	5 Å
Dynamic mode	No
Mutated residues	LC455R, FC486A, NC487K, EC484R, YC489V, FC490K
Energy difference between WT (input) and mutated protein (by FoldX)	2.44305 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.1282
• Maximal score value: 1.738
• Average score: -0.2389
• Total score value: -46.5807

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
333	T	C	-0.3035
334	N	C	-0.9920
335	L	C	1.3270
336	C	C	0.3527
337	P	C	-0.0218
338	F	C	0.1387
339	G	C	-0.6386
340	E	C	-1.8831
341	V	C	0.0000
342	F	C	0.0000
343	N	C	-1.2701
344	A	C	-0.2307
345	T	C	-0.4052
346	R	C	-1.8570
347	F	C	0.0000
348	A	C	0.0045
349	S	C	-0.0232
350	V	C	0.0000
351	Y	C	0.1562
352	A	C	0.0371
353	W	C	0.0000
354	N	C	-1.4285
355	R	C	-1.2266
356	K	C	-1.3025
357	R	C	-1.9796
358	I	C	0.0000
359	S	C	-0.3248
360	N	C	-1.2898
361	C	C	0.0000
362	V	C	0.7176
363	A	C	-0.1323
364	D	C	-1.4741
365	Y	C	0.0000
366	S	C	0.1350
367	V	C	1.7380
368	L	C	0.0000
369	Y	C	1.0797
370	N	C	-1.0430
371	S	C	-0.2958
372	A	C	0.0061
373	S	C	-0.1519
374	F	C	0.2138
375	S	C	-0.1517
376	T	C	0.0480
377	F	C	0.3447
378	K	C	-1.1161
379	C	C	0.0131
380	Y	C	0.4301
381	G	C	-0.3033
382	V	C	0.2238
383	S	C	-0.0363
384	P	C	-0.0605
385	T	C	-0.3870
386	K	C	-1.7129
387	L	C	0.0000
388	N	C	-0.8262
389	D	C	-1.7592
390	L	C	0.3638
391	C	C	0.4098
392	F	C	0.2746
393	T	C	0.0000
394	N	C	-0.1758
395	V	C	0.0000
396	Y	C	-0.0700
397	A	C	0.0000
398	D	C	0.0000
399	S	C	0.0000
400	F	C	0.0000
401	V	C	0.0000
402	I	C	0.0000
403	R	C	-0.2624
404	G	C	-0.3987
405	D	C	-1.3452
406	E	C	0.0000
407	V	C	-0.1275
408	R	C	-1.8305
409	Q	C	-0.4739
410	I	C	0.0000
411	A	C	-0.0030
412	P	C	-0.3343
413	G	C	-0.5572
414	Q	C	-0.5419
415	T	C	-0.1656
416	G	C	-0.2865
417	K	C	-1.0992
418	I	C	0.0000
419	A	C	0.0000
420	D	C	-0.3227
421	Y	C	0.1850
422	N	C	0.0000
423	Y	C	0.0000
424	K	C	-0.5408
425	L	C	0.0000
426	P	C	-0.3687
427	D	C	-2.1282
428	D	C	-2.1159
429	F	C	0.0000
430	T	C	-0.1233
431	G	C	0.0000
432	C	C	0.0000
433	V	C	0.0000
434	I	C	0.0000
435	A	C	0.0000
436	W	C	0.0631
437	N	C	-0.4061
438	S	C	0.0000
439	N	C	-0.4797
440	N	C	-1.0368
441	L	C	1.3163
442	D	C	0.0000
443	S	C	-0.3503
444	K	C	-1.3942
445	V	C	1.3726
446	G	C	-0.1395
447	G	C	0.0000
448	N	C	-0.0931
449	Y	C	1.0594
450	N	C	-1.0329
451	Y	C	0.0000
452	L	C	0.3652
453	Y	C	0.0000
454	R	C	0.0000
455	R	C	-1.0808	mutated: LC455R
456	F	C	0.0876
457	R	C	-0.6365
458	K	C	-1.8108
459	S	C	-0.7586
460	N	C	-1.3370
461	L	C	0.0000
462	K	C	-1.7192
463	P	C	-0.3625
464	F	C	0.5104
465	E	C	-0.5867
466	R	C	-1.4662
467	D	C	0.0000
468	I	C	1.2104
469	S	C	0.0874
470	T	C	-0.4264
471	E	C	-1.7724
472	I	C	0.0538
473	Y	C	0.2587
474	Q	C	-0.7266
475	A	C	-0.3050
476	G	C	-0.3786
477	S	C	-0.2557
478	T	C	-0.1565
479	P	C	-0.2530
480	C	C	-0.2006
481	N	C	-1.3449
482	G	C	-0.3794
483	V	C	1.3461
484	R	C	-1.5931	mutated: EC484R
485	G	C	-0.7904
486	A	C	-0.3375	mutated: FC486A
487	K	C	-1.6896	mutated: NC487K
488	C	C	-0.4293
489	V	C	0.3029	mutated: YC489V
490	K	C	-1.1467	mutated: FC490K
491	P	C	0.0000
492	L	C	0.0000
493	Q	C	-0.3873
494	S	C	-0.1354
495	Y	C	0.0000
496	G	C	-0.0647
497	F	C	0.0000
498	Q	C	-0.4869
499	P	C	-0.2472
500	T	C	-0.1461
501	N	C	-0.3599
502	G	C	-0.1849
503	V	C	1.6505
504	G	C	0.2074
505	Y	C	0.3967
506	Q	C	-0.0526
507	P	C	0.0000
508	Y	C	0.0839
509	R	C	0.0000
510	V	C	0.0000
511	V	C	0.0000
512	V	C	0.0000
513	L	C	0.0000
514	S	C	-0.0208
515	F	C	0.0000
516	E	C	-0.4525
517	L	C	1.6982
518	L	C	1.6525
519	H	C	-0.7005
520	A	C	-0.2045
521	P	C	-0.2456
522	A	C	0.0010
523	T	C	-0.0147
524	V	C	0.0000
525	C	C	0.3766
526	G	C	-0.1342
527	P	C	-0.2677