Project name: SH3_V141H

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA141H
Energy difference between WT (input) and mutated protein (by FoldX)	1.43051 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 0.143
• Average score: -1.1137
• Total score value: -63.4795

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	-0.1430
86	L	A	-0.1453
87	F	A	-0.0553
88	V	A	-0.3514
89	A	A	0.0000
90	L	A	-0.1431
91	Y	A	-0.5734
92	D	A	-2.5594
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7217
105	K	A	-2.3980
106	G	A	-1.7219
107	E	A	-1.2750
108	K	A	-1.0601
109	F	A	0.0000
110	Q	A	-0.5242
111	I	A	-0.0726
112	L	A	0.1430
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1529
122	A	A	0.0000
123	R	A	-1.7076
124	S	A	0.0000
125	L	A	0.1043
126	T	A	-0.4419
127	T	A	-0.8038
128	G	A	-1.3446
129	E	A	-2.2334
130	T	A	-1.6879
131	G	A	-1.4991
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1501
137	Y	A	-0.1232
138	V	A	0.0000
139	A	A	-0.3051
140	P	A	-0.5966
141	H	A	-0.9876	mutated: VA141H