Project name: SH3_Y93C

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA93C
Energy difference between WT (input) and mutated protein (by FoldX)	4.44073 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1571
• Maximal score value: 1.7964
• Average score: -1.017
• Total score value: -57.9681

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9277
88	V	A	0.4362
89	A	A	0.0000
90	L	A	-0.1466
91	Y	A	-0.5799
92	D	A	-2.5816
93	C	A	-2.0000	mutated: YA93C
94	E	A	-2.6983
95	A	A	-2.7008
96	R	A	-3.0345
97	T	A	-2.7117
98	E	A	-3.1571
99	D	A	-3.1338
100	D	A	-2.2606
101	L	A	0.0000
102	S	A	-2.2660
103	F	A	0.0000
104	H	A	-2.7428
105	K	A	-2.4113
106	G	A	-1.4592
107	E	A	-1.3102
108	K	A	-0.6438
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0573
112	L	A	0.1425
113	N	A	-0.8975
114	S	A	-1.1933
115	S	A	-1.6044
116	E	A	-2.5721
117	G	A	-2.1525
118	D	A	-2.4758
119	W	A	-1.1893
120	W	A	-1.1100
121	E	A	-1.1958
122	A	A	0.0000
123	R	A	-1.7161
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.7199
131	G	A	-1.5721
132	Y	A	-0.9685
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9297
136	N	A	-1.1421
137	Y	A	-0.1038
138	V	A	0.0000
139	A	A	0.4236
140	P	A	0.7757
141	V	A	1.7964