Project name: SH3_G117V

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA117V
Energy difference between WT (input) and mutated protein (by FoldX)	5.23664 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -0.8396
• Total score value: -47.8584

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9237
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5084
111	I	A	-0.0552
112	L	A	0.1450
113	N	A	-0.6124
114	S	A	-0.6552
115	S	A	-0.9354
116	E	A	-1.5448
117	V	A	-0.0693	mutated: GA117V
118	D	A	-1.4645
119	W	A	-0.5714
120	W	A	-0.6499
121	E	A	-0.9552
122	A	A	0.0000
123	R	A	-1.7150
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.5085
132	Y	A	-0.8911
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.6705
136	N	A	-1.1629
137	Y	A	-0.1335
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964