Project name: SH3_Y137R

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA137R
Energy difference between WT (input) and mutated protein (by FoldX)	1.1285 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7964
• Average score: -1.0454
• Total score value: -59.5906

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.7822
88	V	A	0.2427
89	A	A	0.0000
90	L	A	-0.5400
91	Y	A	-0.8852
92	D	A	-2.7520
93	Y	A	-2.1547
94	E	A	-2.6524
95	A	A	-2.6296
96	R	A	-2.9869
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2108
103	F	A	0.0000
104	H	A	-2.7319
105	K	A	-2.5719
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5085
111	I	A	-0.0554
112	L	A	0.1440
113	N	A	-0.9101
114	S	A	-1.2149
115	S	A	-1.6256
116	E	A	-2.6048
117	G	A	-2.2133
118	D	A	-2.5251
119	W	A	-1.3682
120	W	A	-1.1216
121	E	A	-1.1840
122	A	A	0.0000
123	R	A	-1.7149
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6927
131	G	A	-1.5132
132	Y	A	-0.9031
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-1.3580
136	N	A	-1.7598
137	R	A	-1.3624	mutated: YA137R
138	V	A	0.0000
139	A	A	0.1562
140	P	A	0.7757
141	V	A	1.7964