Project name: 10-8pig [mutate: SL24I, QL26S, GL51A, RL55L, AL56L, SH166G, FH215L]

Chain sequence(s)	H: QVQLVQSGAEVKKPGASVKVSCKASGYTFTDYSVHWVRQAPGQGLEWMGVINTYSGNTDYAQKFQGRVTMTRDTSTSTVYMELSSLRSEDTAVYYCARGYGSWFAYWGQGTTLT; L: DIQMTQSPSSLSASVGDRVTITCSVQSSISSSGLHWYQQKPEKAPKSLIFGTSNRASGVPSRFSGSGSGTDFTLTISSLQPEDFATYYCQHWSSYPLTFGAGTKLEIKRTV
Distance of aggregation	5 Å
Dynamic mode	No
Mutated residues	SL24I, QL26S, GL51A, RL55L, AL56L, SH166G, FH215L
Energy difference between WT (input) and mutated protein (by FoldX)	2.95534 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -2.1698
• Maximal score value: 1.7596
• Average score: -0.2424
• Total score value: -54.3035

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
1	D	L	-1.7503
2	I	L	-0.3313
3	Q	L	-1.1592
4	M	L	0.0000
5	T	L	-0.0705
6	Q	L	0.0000
7	S	L	-0.2287
8	P	L	-0.1961
9	S	L	-0.2690
10	S	L	-0.4352
11	L	L	0.1302
12	S	L	-0.1736
13	A	L	-0.0403
14	S	L	-0.0562
15	V	L	1.0238
16	G	L	-0.1920
17	D	L	-1.2458
18	R	L	-2.0152
19	V	L	0.0000
20	T	L	-0.0726
21	I	L	0.0000
22	T	L	-0.0219
23	C	L	0.0000
24	I	L	0.6718	mutated: SL24I
25	V	L	0.0000
26	S	L	-0.2521	mutated: QL26S
27	S	L	-0.2914
28	S	L	-0.2256
29	I	L	0.1387
30	S	L	-0.2110
31	S	L	-0.1662
32	S	L	-0.2168
33	G	L	0.0000
34	L	L	0.0000
35	H	L	0.0000
36	W	L	0.0000
37	Y	L	0.0000
38	Q	L	0.0000
39	Q	L	-0.2006
40	K	L	-0.4723
41	P	L	-0.6094
42	E	L	-2.1698
43	K	L	-2.0324
44	A	L	-0.2955
45	P	L	0.0000
46	K	L	-1.1264
47	S	L	0.0000
48	L	L	0.0000
49	I	L	0.0000
50	F	L	0.3525
51	A	L	0.0450	mutated: GL51A
52	T	L	0.0000
53	S	L	-0.4130
54	N	L	-1.2143
55	L	L	0.3098	mutated: RL55L
56	L	L	0.3629	mutated: AL56L
57	S	L	-0.2406
58	G	L	-0.4679
59	V	L	0.0966
60	P	L	-0.1199
61	S	L	-0.3021
62	R	L	-0.4095
63	F	L	0.0000
64	S	L	-0.1343
65	G	L	-0.1404
66	S	L	-0.2484
67	G	L	-0.3213
68	S	L	-0.2844
69	G	L	-0.1651
70	T	L	-0.3255
71	D	L	-1.3610
72	F	L	0.0000
73	T	L	-0.0135
74	L	L	0.0000
75	T	L	-0.0229
76	I	L	0.0000
77	S	L	-0.3531
78	S	L	-0.2335
79	L	L	0.0000
80	Q	L	-0.3688
81	P	L	-0.6638
82	E	L	-1.8659
83	D	L	0.0000
84	F	L	1.1000
85	A	L	0.0000
86	T	L	0.0000
87	Y	L	0.0000
88	Y	L	0.1385
89	C	L	0.0000
90	Q	L	0.0000
91	H	L	0.0000
92	W	L	0.3445
93	S	L	-0.0637
94	S	L	0.0082
95	Y	L	1.2664
96	P	L	0.3207
97	L	L	0.0000
98	T	L	0.0255
99	F	L	0.2013
100	G	L	0.0000
101	A	L	0.0585
102	G	L	0.0000
103	T	L	0.0000
104	K	L	-1.7279
105	L	L	0.0000
106	E	L	-1.1785
107	I	L	-0.2554
108	K	L	-1.9915
109	R	L	-2.1691
110	T	L	-0.0808
111	V	L	1.7596
112	Q	H	-1.1617
113	V	H	-0.2881
114	Q	H	-0.9162
115	L	H	0.0000
116	V	H	1.6481
117	Q	H	0.0000
118	S	H	-0.1492
119	G	H	-0.4672
120	A	H	-0.2136
121	E	H	-0.6306
122	V	H	1.5065
123	K	H	-0.3896
124	K	H	-1.8220
125	P	H	-0.7969
126	G	H	-0.5390
127	A	H	-0.1020
128	S	H	-0.1526
129	V	H	0.0000
130	K	H	-1.8016
131	V	H	0.0000
132	S	H	-0.0142
133	C	H	0.0000
134	K	H	-0.8370
135	A	H	0.0000
136	S	H	-0.2994
137	G	H	-0.4336
138	Y	H	0.1089
139	T	H	-0.0326
140	F	H	0.0000
141	T	H	-0.1555
142	D	H	-1.7159
143	Y	H	0.0826
144	S	H	-0.0464
145	V	H	0.0000
146	H	H	0.0000
147	W	H	0.0000
148	V	H	0.0000
149	R	H	0.0000
150	Q	H	-0.4930
151	A	H	-0.1302
152	P	H	-0.3395
153	G	H	-0.5132
155	G	H	-0.3238
156	L	H	0.0000
157	E	H	-0.2848
158	W	H	0.0000
159	M	H	0.0000
160	G	H	0.0000
161	V	H	0.1867
162	I	H	0.0000
163	N	H	-0.7299
164	T	H	0.0000
165	Y	H	1.2568
166	G	H	-0.0785	mutated: SH166G
167	G	H	-0.3951
168	N	H	-1.2962
169	T	H	-0.5864
170	D	H	-1.7350
171	Y	H	-0.0164
172	A	H	0.0000
173	Q	H	-1.6365
174	K	H	-1.9200
175	F	H	0.0000
176	Q	H	-1.4099
177	G	H	-0.7268
178	R	H	-0.3179
179	V	H	0.0000
180	T	H	-0.0552
181	M	H	0.0000
182	T	H	-0.1407
183	R	H	-0.7539
184	D	H	-0.9031
185	T	H	-0.2467
186	S	H	-0.2284
187	T	H	-0.0776
188	S	H	-0.0629
189	T	H	0.0000
190	V	H	0.0000
191	Y	H	0.2111
192	M	H	0.0000
193	E	H	-0.9339
194	L	H	0.0000
195	S	H	-0.1659
196	S	H	-0.2583
197	L	H	0.0000
198	R	H	-1.4294
199	S	H	-0.7855
200	E	H	-1.7937
201	D	H	0.0000
202	T	H	-0.0363
203	A	H	0.0000
204	V	H	0.3107
205	Y	H	0.0000
206	Y	H	0.0000
207	C	H	0.0000
208	A	H	0.0000
209	R	H	-0.2035
210	G	H	0.0000
211	Y	H	1.2720
212	G	H	-0.0328
213	S	H	-0.2541
214	W	H	0.0000
215	L	H	0.0000	mutated: FH215L
216	A	H	0.0627
217	Y	H	0.2907
218	W	H	0.0000
219	G	H	-0.3138
220	Q	H	-1.2185
221	G	H	-0.2746
222	T	H	0.0000
223	T	H	0.0177
224	L	H	0.0000
225	T	H	0.1178