Project name: SH3_L125P

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	LA125P
Energy difference between WT (input) and mutated protein (by FoldX)	4.90861 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8306
• Average score: -1.0506
• Total score value: -59.8829

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5578
86	L	A	0.7022
87	F	A	0.8064
88	V	A	0.2814
89	A	A	0.0000
90	L	A	-0.1553
91	Y	A	-0.5747
92	D	A	-2.5596
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7323
105	K	A	-2.4108
106	G	A	-1.4674
107	E	A	-1.6055
108	K	A	-1.1100
109	F	A	0.0000
110	Q	A	-0.8756
111	I	A	-0.0920
112	L	A	0.1120
113	N	A	-0.9012
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0686
121	E	A	-1.1710
122	A	A	0.0000
123	R	A	-2.0987
124	S	A	0.0000
125	P	A	-1.3155	mutated: LA125P
126	T	A	-1.1546
127	T	A	-1.2175
128	G	A	-1.7848
129	E	A	-2.4607
130	T	A	-1.8607
131	G	A	-1.5019
132	Y	A	-0.8690
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1246
138	V	A	0.0000
139	A	A	0.4305
140	P	A	0.7966
141	V	A	1.8306