Project name: SH3_W119T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA119T
Energy difference between WT (input) and mutated protein (by FoldX)	1.56141 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1032
• Maximal score value: 1.7964
• Average score: -1.0116
• Total score value: -57.6587

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9240
88	V	A	0.4316
89	A	A	0.0000
90	L	A	-0.1470
91	Y	A	-0.5669
92	D	A	-2.5455
93	Y	A	-1.9070
94	E	A	-2.6312
95	A	A	-2.6225
96	R	A	-2.9819
97	T	A	-2.6608
98	E	A	-3.1032
99	D	A	-3.0440
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1973
103	F	A	0.0000
104	H	A	-2.7210
105	K	A	-2.4006
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5094
111	I	A	-0.0578
112	L	A	0.1415
113	N	A	-0.9637
114	S	A	-1.2895
115	S	A	-1.6634
116	E	A	-2.6598
117	G	A	-2.2885
118	D	A	-2.7045
119	T	A	-1.6301	mutated: WA119T
120	W	A	-1.3218
121	E	A	-1.2798
122	A	A	0.0000
123	R	A	-1.7158
124	S	A	0.0000
125	L	A	0.0592
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.6932
131	G	A	-1.5613
132	Y	A	-1.0325
133	I	A	0.0000
134	P	A	-0.7905
135	S	A	-1.1032
136	N	A	-1.2377
137	Y	A	-0.1828
138	V	A	0.0000
139	A	A	0.4162
140	P	A	0.7757
141	V	A	1.7964