| Chain sequence(s) |
A: RAARGPLLPNRPFTTVWNANTQWCLERHGVDVDVSVFDVVANPGQTFRGPDMTIFYSSQLGTYPYYTPTGEPVFGGLPQNASLIAHLARTFQDILAAIPAPDFSGLAVIDWEAWRPRWAFNWDTKDIYRQRSRALVQAQHPDWPAPQVEAVAQDQFQGAARAWMAGTLQLGRALRPRGLWGFYGFPDCYNYDFLSPNYTGQCPSGIRAQNDQLGWLWGQSRALYPSIYMPAVLEGTGKSQMYVQHRVAEAFRVAVAAGDPNLPVLPYVQIFYDTTNHFLPLDELEHSLGESAAQGAAGVVLWVSWENTRTKESCQAIKEYMDTTLGPFILNVTSGALLCSQALCSGHGRCVRRTSHPKALLLLNPASFSIQLTPGGGPLSLRGALSLEDQAQMAVEFKCRCYPGWQAPWCERKSMWT |
| Distance of aggregation | 10 Å |
| Dynamic mode | No |
| Mutated residues | AA22S |
| Energy difference between WT (input) and mutated protein (by FoldX) | -0.234833 kcal/mol
Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive. |
The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.
| residue index | residue name | chain | Aggrescan3D score | mutation |
|---|---|---|---|---|
| residue index | residue name | chain | Aggrescan3D score | |
| 20 | R | A | -2.2183 | |
| 21 | A | A | -1.6205 | |
| 22 | S | A | -1.0970 | mutated: AA22S |
| 23 | R | A | -1.1779 | |
| 24 | G | A | -0.7316 | |
| 25 | P | A | -0.7466 | |
| 26 | L | A | 0.0000 | |
| 27 | L | A | -0.5658 | |
| 28 | P | A | -0.9195 | |
| 29 | N | A | -1.7167 | |
| 30 | R | A | -1.1601 | |
| 31 | P | A | 0.0000 | |
| 32 | F | A | 0.0000 | |
| 33 | T | A | 0.0000 | |
| 34 | T | A | 0.0000 | |
| 35 | V | A | 0.0000 | |
| 36 | W | A | 0.0000 | |
| 37 | N | A | 0.0000 | |
| 38 | A | A | 0.0000 | |
| 39 | N | A | -0.3192 | |
| 40 | T | A | 0.0000 | |
| 41 | Q | A | -1.4135 | |
| 42 | W | A | -1.8238 | |
| 43 | C | A | 0.0000 | |
| 44 | L | A | -1.7969 | |
| 45 | E | A | -2.7969 | |
| 46 | R | A | -3.1600 | |
| 47 | H | A | -2.8141 | |
| 48 | G | A | -2.0121 | |
| 49 | V | A | 0.0000 | |
| 50 | D | A | -2.2872 | |
| 51 | V | A | 0.0000 | |
| 52 | D | A | -1.4988 | |
| 53 | V | A | 0.0056 | |
| 54 | S | A | 0.0063 | |
| 55 | V | A | 0.2731 | |
| 56 | F | A | 0.0000 | |
| 57 | D | A | -0.1823 | |
| 58 | V | A | 0.0000 | |
| 59 | V | A | 1.1559 | |
| 60 | A | A | 0.2620 | |
| 61 | N | A | -0.1733 | |
| 62 | P | A | 0.0188 | |
| 63 | G | A | -0.5806 | |
| 64 | Q | A | -0.7661 | |
| 65 | T | A | -0.1382 | |
| 66 | F | A | 0.6692 | |
| 67 | R | A | -1.2823 | |
| 68 | G | A | -0.7961 | |
| 69 | P | A | -1.1175 | |
| 70 | D | A | -1.0217 | |
| 71 | M | A | -0.4981 | |
| 72 | T | A | -0.3153 | |
| 73 | I | A | -0.1009 | |
| 74 | F | A | 0.0000 | |
| 75 | Y | A | -0.0555 | |
| 76 | S | A | -0.4310 | |
| 77 | S | A | -0.3715 | |
| 78 | Q | A | -0.7141 | |
| 79 | L | A | 0.0000 | |
| 80 | G | A | -0.6657 | |
| 81 | T | A | 0.0043 | |
| 82 | Y | A | 0.0000 | |
| 83 | P | A | 0.0000 | |
| 84 | Y | A | 0.2001 | |
| 85 | Y | A | 0.0000 | |
| 86 | T | A | -0.6790 | |
| 87 | P | A | -0.6085 | |
| 88 | T | A | -0.7493 | |
| 89 | G | A | -1.0081 | |
| 90 | E | A | -1.7030 | |
| 91 | P | A | -0.5977 | |
| 92 | V | A | 0.7239 | |
| 93 | F | A | 1.3435 | |
| 94 | G | A | 0.1307 | |
| 95 | G | A | 0.0000 | |
| 96 | L | A | 0.0000 | |
| 97 | P | A | 0.0000 | |
| 98 | Q | A | -1.1623 | |
| 99 | N | A | -1.1072 | |
| 100 | A | A | 0.1151 | |
| 101 | S | A | 0.6338 | |
| 102 | L | A | 0.9674 | |
| 103 | I | A | 2.0145 | |
| 104 | A | A | 0.7206 | |
| 105 | H | A | 0.0000 | |
| 106 | L | A | 0.6437 | |
| 107 | A | A | -0.0861 | |
| 108 | R | A | -1.5887 | |
| 109 | T | A | 0.0000 | |
| 110 | F | A | 0.0502 | |
| 111 | Q | A | -1.0072 | |
| 112 | D | A | -0.7563 | |
| 113 | I | A | 0.0000 | |
| 114 | L | A | 0.4291 | |
| 115 | A | A | -0.0712 | |
| 116 | A | A | -0.4264 | |
| 117 | I | A | 0.0000 | |
| 118 | P | A | -0.6063 | |
| 119 | A | A | -0.7161 | |
| 120 | P | A | -1.3613 | |
| 121 | D | A | -2.5206 | |
| 122 | F | A | 0.0000 | |
| 123 | S | A | -1.6086 | |
| 124 | G | A | 0.0000 | |
| 125 | L | A | 0.0000 | |
| 126 | A | A | 0.0000 | |
| 127 | V | A | 0.0000 | |
| 128 | I | A | 0.0000 | |
| 129 | D | A | -0.6765 | |
| 130 | W | A | 0.0000 | |
| 131 | E | A | -1.9073 | |
| 132 | A | A | -0.9661 | |
| 133 | W | A | 0.0000 | |
| 134 | R | A | -0.8628 | |
| 135 | P | A | 0.0000 | |
| 136 | R | A | -0.8339 | |
| 137 | W | A | -0.5983 | |
| 138 | A | A | -0.1963 | |
| 139 | F | A | 0.0000 | |
| 140 | N | A | 0.0000 | |
| 141 | W | A | -0.7592 | |
| 142 | D | A | -1.7967 | |
| 143 | T | A | -1.0500 | |
| 144 | K | A | -1.2529 | |
| 145 | D | A | -1.2646 | |
| 146 | I | A | -0.9215 | |
| 147 | Y | A | 0.0000 | |
| 148 | R | A | -1.5988 | |
| 149 | Q | A | -1.8885 | |
| 150 | R | A | -1.5102 | |
| 151 | S | A | 0.0000 | |
| 152 | R | A | -2.2474 | |
| 153 | A | A | -1.3689 | |
| 154 | L | A | -0.9588 | |
| 155 | V | A | 0.0000 | |
| 156 | Q | A | -1.7829 | |
| 157 | A | A | -1.1626 | |
| 158 | Q | A | -1.6404 | |
| 159 | H | A | -1.6223 | |
| 160 | P | A | -1.6647 | |
| 161 | D | A | -2.1446 | |
| 162 | W | A | -1.2357 | |
| 163 | P | A | -1.0360 | |
| 164 | A | A | -1.0878 | |
| 165 | P | A | -1.1214 | |
| 166 | Q | A | -1.3178 | |
| 167 | V | A | 0.0000 | |
| 168 | E | A | -2.6868 | |
| 169 | A | A | -1.6853 | |
| 170 | V | A | -1.5315 | |
| 171 | A | A | 0.0000 | |
| 172 | Q | A | -2.3172 | |
| 173 | D | A | -2.6424 | |
| 174 | Q | A | -2.3750 | |
| 175 | F | A | 0.0000 | |
| 176 | Q | A | -1.7682 | |
| 177 | G | A | -1.8007 | |
| 178 | A | A | -1.4176 | |
| 179 | A | A | 0.0000 | |
| 180 | R | A | -1.7517 | |
| 181 | A | A | -0.7879 | |
| 182 | W | A | 0.0000 | |
| 183 | M | A | 0.0000 | |
| 184 | A | A | -0.7425 | |
| 185 | G | A | -0.5612 | |
| 186 | T | A | 0.0000 | |
| 187 | L | A | 0.0000 | |
| 188 | Q | A | -1.0501 | |
| 189 | L | A | -0.1121 | |
| 190 | G | A | 0.0000 | |
| 191 | R | A | -1.0419 | |
| 192 | A | A | -0.3837 | |
| 193 | L | A | -0.2587 | |
| 194 | R | A | 0.0000 | |
| 195 | P | A | -1.7396 | |
| 196 | R | A | -2.6542 | |
| 197 | G | A | 0.0000 | |
| 198 | L | A | 0.0000 | |
| 199 | W | A | 0.0000 | |
| 200 | G | A | 0.0000 | |
| 201 | F | A | 0.0000 | |
| 202 | Y | A | 0.0000 | |
| 203 | G | A | -0.4639 | |
| 204 | F | A | 0.0000 | |
| 205 | P | A | 0.0000 | |
| 206 | D | A | -0.3454 | |
| 207 | C | A | 0.0000 | |
| 208 | Y | A | 0.0000 | |
| 209 | N | A | 0.0000 | |
| 210 | Y | A | 0.0969 | |
| 211 | D | A | -1.3125 | |
| 212 | F | A | 0.0000 | |
| 213 | L | A | 1.0466 | |
| 214 | S | A | -0.2408 | |
| 215 | P | A | -0.7340 | |
| 216 | N | A | -1.3755 | |
| 217 | Y | A | -0.8572 | |
| 218 | T | A | -0.9999 | |
| 219 | G | A | 0.0000 | |
| 220 | Q | A | -1.4919 | |
| 221 | C | A | 0.0000 | |
| 222 | P | A | -0.8860 | |
| 223 | S | A | -0.7126 | |
| 224 | G | A | -0.8146 | |
| 225 | I | A | 0.0000 | |
| 226 | R | A | -1.7717 | |
| 227 | A | A | -1.3927 | |
| 228 | Q | A | -1.5096 | |
| 229 | N | A | 0.0000 | |
| 230 | D | A | -2.4293 | |
| 231 | Q | A | -2.0440 | |
| 232 | L | A | 0.0000 | |
| 233 | G | A | -1.0208 | |
| 234 | W | A | -0.6267 | |
| 235 | L | A | 0.0000 | |
| 236 | W | A | 0.0000 | |
| 237 | G | A | -0.6583 | |
| 238 | Q | A | -0.5707 | |
| 239 | S | A | 0.0000 | |
| 240 | R | A | -1.1553 | |
| 241 | A | A | 0.0000 | |
| 242 | L | A | 0.0000 | |
| 243 | Y | A | 0.0000 | |
| 244 | P | A | 0.0000 | |
| 245 | S | A | -0.0450 | |
| 246 | I | A | 0.0000 | |
| 247 | Y | A | 0.0419 | |
| 248 | M | A | 0.0000 | |
| 249 | P | A | 0.2898 | |
| 250 | A | A | 0.0748 | |
| 251 | V | A | 1.4560 | |
| 252 | L | A | 0.0000 | |
| 253 | E | A | -1.1762 | |
| 254 | G | A | -0.9725 | |
| 255 | T | A | -0.6928 | |
| 256 | G | A | -0.9670 | |
| 257 | K | A | -0.6435 | |
| 258 | S | A | 0.0000 | |
| 259 | Q | A | -0.9154 | |
| 260 | M | A | -0.5393 | |
| 261 | Y | A | 0.0000 | |
| 262 | V | A | 0.0000 | |
| 263 | Q | A | -0.7283 | |
| 264 | H | A | -0.6508 | |
| 265 | R | A | 0.0000 | |
| 266 | V | A | 0.0000 | |
| 267 | A | A | -0.3404 | |
| 268 | E | A | 0.0000 | |
| 269 | A | A | 0.0000 | |
| 270 | F | A | 0.2888 | |
| 271 | R | A | -0.1807 | |
| 272 | V | A | 0.0000 | |
| 273 | A | A | 0.0000 | |
| 274 | V | A | 1.3559 | |
| 275 | A | A | 0.3294 | |
| 276 | A | A | -0.1948 | |
| 277 | G | A | -0.3447 | |
| 278 | D | A | 0.0000 | |
| 279 | P | A | -0.1932 | |
| 280 | N | A | -0.9494 | |
| 281 | L | A | 0.0000 | |
| 282 | P | A | 0.0000 | |
| 283 | V | A | 0.0000 | |
| 284 | L | A | 0.0000 | |
| 285 | P | A | 0.0000 | |
| 286 | Y | A | 0.0000 | |
| 287 | V | A | 0.0000 | |
| 288 | Q | A | 0.0000 | |
| 289 | I | A | 0.0000 | |
| 290 | F | A | 0.0000 | |
| 291 | Y | A | 0.0000 | |
| 292 | D | A | -0.5543 | |
| 293 | T | A | -0.8296 | |
| 294 | T | A | -1.1340 | |
| 295 | N | A | -1.6918 | |
| 296 | H | A | -1.4528 | |
| 297 | F | A | -0.2863 | |
| 298 | L | A | 0.0000 | |
| 299 | P | A | -0.3285 | |
| 300 | L | A | 0.4707 | |
| 301 | D | A | -1.3349 | |
| 302 | E | A | 0.0000 | |
| 303 | L | A | 0.0000 | |
| 304 | E | A | -0.8248 | |
| 305 | H | A | -1.0572 | |
| 306 | S | A | 0.0000 | |
| 307 | L | A | 0.0000 | |
| 308 | G | A | 0.0000 | |
| 309 | E | A | 0.0000 | |
| 310 | S | A | 0.0000 | |
| 311 | A | A | 0.0000 | |
| 312 | A | A | 0.0000 | |
| 313 | Q | A | -0.3535 | |
| 314 | G | A | 0.0000 | |
| 315 | A | A | 0.0000 | |
| 316 | A | A | 0.0000 | |
| 317 | G | A | 0.0000 | |
| 318 | V | A | 0.0000 | |
| 319 | V | A | 0.0000 | |
| 320 | L | A | 0.0000 | |
| 321 | W | A | 0.4079 | |
| 322 | V | A | 0.0000 | |
| 323 | S | A | 0.0000 | |
| 324 | W | A | -0.4817 | |
| 325 | E | A | -2.0950 | |
| 326 | N | A | 0.0000 | |
| 327 | T | A | 0.0000 | |
| 328 | R | A | -2.9548 | |
| 329 | T | A | -2.7614 | |
| 330 | K | A | -3.2402 | |
| 331 | E | A | -3.1309 | |
| 332 | S | A | -2.4989 | |
| 333 | C | A | 0.0000 | |
| 334 | Q | A | -2.6522 | |
| 335 | A | A | -1.9483 | |
| 336 | I | A | 0.0000 | |
| 337 | K | A | -2.6542 | |
| 338 | E | A | -2.6797 | |
| 339 | Y | A | -1.3443 | |
| 340 | M | A | 0.0000 | |
| 341 | D | A | -1.8781 | |
| 342 | T | A | -0.8668 | |
| 343 | T | A | -0.2017 | |
| 344 | L | A | 0.0000 | |
| 345 | G | A | 0.0000 | |
| 346 | P | A | -0.1065 | |
| 347 | F | A | 0.0000 | |
| 348 | I | A | 0.0000 | |
| 349 | L | A | 0.3925 | |
| 350 | N | A | 0.0349 | |
| 351 | V | A | 0.0000 | |
| 352 | T | A | 0.0000 | |
| 353 | S | A | 0.0575 | |
| 354 | G | A | 0.0000 | |
| 355 | A | A | 0.0000 | |
| 356 | L | A | -0.4637 | |
| 357 | L | A | -0.2042 | |
| 358 | C | A | 0.0000 | |
| 359 | S | A | 0.0000 | |
| 360 | Q | A | -1.3548 | |
| 361 | A | A | -0.3048 | |
| 362 | L | A | -0.5801 | |
| 363 | C | A | 0.0000 | |
| 364 | S | A | -1.3100 | |
| 365 | G | A | -1.3266 | |
| 366 | H | A | -1.5839 | |
| 367 | G | A | 0.0000 | |
| 368 | R | A | -0.6407 | |
| 369 | C | A | 0.0000 | |
| 370 | V | A | -0.4338 | |
| 371 | R | A | 0.0000 | |
| 372 | R | A | -1.0360 | |
| 373 | T | A | -0.9526 | |
| 374 | S | A | -0.7455 | |
| 375 | H | A | -1.1451 | |
| 376 | P | A | -1.4895 | |
| 377 | K | A | -2.0570 | |
| 378 | A | A | -1.0026 | |
| 379 | L | A | -0.2992 | |
| 380 | L | A | 0.0000 | |
| 381 | L | A | 0.1618 | |
| 382 | L | A | 0.0000 | |
| 383 | N | A | -0.8734 | |
| 384 | P | A | -0.5393 | |
| 385 | A | A | -0.3523 | |
| 386 | S | A | -0.6902 | |
| 387 | F | A | 0.0000 | |
| 388 | S | A | -0.9307 | |
| 389 | I | A | -0.3298 | |
| 390 | Q | A | -0.8202 | |
| 391 | L | A | -0.0762 | |
| 392 | T | A | -0.3109 | |
| 393 | P | A | -0.6506 | |
| 394 | G | A | -0.7897 | |
| 395 | G | A | -0.8208 | |
| 396 | G | A | -0.6353 | |
| 397 | P | A | -0.4254 | |
| 398 | L | A | -0.2654 | |
| 399 | S | A | -0.2524 | |
| 400 | L | A | -0.4899 | |
| 401 | R | A | -1.9077 | |
| 402 | G | A | -1.1480 | |
| 403 | A | A | -0.2285 | |
| 404 | L | A | 0.1167 | |
| 405 | S | A | -0.1849 | |
| 406 | L | A | 0.3346 | |
| 407 | E | A | -1.6712 | |
| 408 | D | A | -1.2238 | |
| 409 | Q | A | -0.7174 | |
| 410 | A | A | -0.8303 | |
| 411 | Q | A | -1.3345 | |
| 412 | M | A | 0.0000 | |
| 413 | A | A | -0.3831 | |
| 414 | V | A | 0.3263 | |
| 415 | E | A | -0.6564 | |
| 416 | F | A | 0.0000 | |
| 417 | K | A | -0.9691 | |
| 418 | C | A | -0.6542 | |
| 419 | R | A | -0.7645 | |
| 420 | C | A | -0.8573 | |
| 421 | Y | A | 0.0000 | |
| 422 | P | A | -0.9702 | |
| 423 | G | A | -0.6855 | |
| 424 | W | A | -1.4842 | |
| 425 | Q | A | -1.6809 | |
| 426 | A | A | -1.1018 | |
| 427 | P | A | -0.7460 | |
| 428 | W | A | -0.7691 | |
| 429 | C | A | 0.0000 | |
| 430 | E | A | -3.0833 | |
| 431 | R | A | -3.4916 | |
| 432 | K | A | -2.7123 | |
| 433 | S | A | -0.8706 | |
| 434 | M | A | 0.9157 | |
| 435 | W | A | 1.4165 | |
| 436 | T | A | 0.4269 |
ALA alanine
ARG arginine
ASN asparagine
ASP aspartic acid
CYS cysteine
GLN glutamine
GLU glutamic acid
GLY glycine
HIS histidine
ILE isoleucine
LEU leucine
LYS lysine
MET methionine
PHE phenylalanine
PRO proline
SER serine
THR threonine
TRP tryptophan
TYR tyrosine
VAL valine