Project name: SH3_E107Q

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA107Q
Energy difference between WT (input) and mutated protein (by FoldX)	1.71186 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1029
• Maximal score value: 1.8005
• Average score: -0.9527
• Total score value: -54.3041

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7949
87	F	A	0.9429
88	V	A	0.4688
89	A	A	0.0000
90	L	A	-0.1340
91	Y	A	-0.5624
92	D	A	-2.5466
93	Y	A	-1.9317
94	E	A	-2.6455
95	A	A	-2.6262
96	R	A	-2.9853
97	T	A	-2.6609
98	E	A	-3.1029
99	D	A	-3.0435
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2054
103	F	A	0.0000
104	H	A	-2.6954
105	K	A	-2.3720
106	G	A	-1.4044
107	Q	A	-1.2051	mutated: EA107Q
108	K	A	-0.5819
109	F	A	0.0000
110	Q	A	-0.5112
111	I	A	-0.0542
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7175
124	S	A	0.0000
125	L	A	0.0546
126	T	A	-0.4592
127	T	A	-0.8138
128	G	A	-1.3630
129	E	A	-2.2362
130	T	A	-1.6919
131	G	A	-1.4971
132	Y	A	-0.8674
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4187
140	P	A	0.7786
141	V	A	1.8005