Project name: SH3_A95E

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	AA95E
Energy difference between WT (input) and mutated protein (by FoldX)	4.74827 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.253
• Maximal score value: 1.7964
• Average score: -0.9923
• Total score value: -56.5632

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9238
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.6102
93	Y	A	-2.0600
94	E	A	-2.9051
95	E	A	-3.1682	mutated: AA95E
96	R	A	-3.2530
97	T	A	-2.8127
98	E	A	-3.2017
99	D	A	-3.1313
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.3882
103	F	A	0.0000
104	H	A	-2.7206
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6426
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0568
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7125
124	S	A	0.0000
125	L	A	0.0607
126	T	A	-0.4715
127	T	A	-0.8176
128	G	A	-1.3526
129	E	A	-2.2262
130	T	A	-1.6841
131	G	A	-1.5545
132	Y	A	-0.9355
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964