Project name: SH3_Y93G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	YA93G
Energy difference between WT (input) and mutated protein (by FoldX)	5.49966 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1594
• Maximal score value: 1.7964
• Average score: -1.0382
• Total score value: -59.177

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9336
88	V	A	0.4446
89	A	A	0.0000
90	L	A	-0.1567
91	Y	A	-0.6203
92	D	A	-2.6742
93	G	A	-2.2013	mutated: YA93G
94	E	A	-2.7989
95	A	A	-2.7571
96	R	A	-3.0594
97	T	A	-2.7138
98	E	A	-3.1594
99	D	A	-3.1379
100	D	A	-2.2910
101	L	A	0.0000
102	S	A	-2.3336
103	F	A	0.0000
104	H	A	-2.7928
105	K	A	-2.4392
106	G	A	-1.4570
107	E	A	-1.3055
108	K	A	-0.6415
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0573
112	L	A	0.1425
113	N	A	-0.8930
114	S	A	-1.1865
115	S	A	-1.6003
116	E	A	-2.5660
117	G	A	-2.1463
118	D	A	-2.4676
119	W	A	-1.1657
120	W	A	-1.1025
121	E	A	-1.1934
122	A	A	0.0000
123	R	A	-1.7161
124	S	A	0.0000
125	L	A	0.0596
126	T	A	-0.4724
127	T	A	-0.8204
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.7211
131	G	A	-1.5712
132	Y	A	-0.9692
133	I	A	0.0000
134	P	A	-0.6351
135	S	A	-0.9190
136	N	A	-1.1248
137	Y	A	-0.0992
138	V	A	0.0000
139	A	A	0.4332
140	P	A	0.7757
141	V	A	1.7964