Project name: SH3_K108H

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	KA108H
Energy difference between WT (input) and mutated protein (by FoldX)	1.17162 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.863
• Average score: -0.9188
• Total score value: -52.3737

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5303
86	L	A	0.9190
87	F	A	1.0474
88	V	A	0.5730
89	A	A	0.0000
90	L	A	-0.0955
91	Y	A	-0.5565
92	D	A	-2.5371
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.6524
105	K	A	-2.2963
106	G	A	-1.2384
107	E	A	-1.0652
108	H	A	-0.2313	mutated: KA108H
109	F	A	0.0000
110	Q	A	-0.4091
111	I	A	-0.0066
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.6370
124	S	A	0.0000
125	L	A	0.2484
126	T	A	-0.3391
127	T	A	-0.7615
128	G	A	-1.3353
129	E	A	-2.2286
130	T	A	-1.6848
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1504
137	Y	A	-0.1374
138	V	A	0.0000
139	A	A	0.4538
140	P	A	0.8121
141	V	A	1.8630