Project name: SH3_F87T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	FA87T
Energy difference between WT (input) and mutated protein (by FoldX)	4.42019 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7165
• Average score: -0.9828
• Total score value: -56.0199

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4700
86	L	A	0.6897
87	T	A	0.0000	mutated: FA87T
88	V	A	0.3149
89	A	A	0.0000
90	L	A	-0.1790
91	Y	A	-0.5750
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7289
105	K	A	-2.4069
106	G	A	-1.4872
107	E	A	-1.3455
108	K	A	-0.7146
109	F	A	0.0000
110	Q	A	-0.5372
111	I	A	-0.0410
112	L	A	0.1785
113	N	A	-0.8542
114	S	A	-1.1444
115	S	A	-1.5847
116	E	A	-2.5469
117	G	A	-2.1204
118	D	A	-2.4266
119	W	A	-1.0708
120	W	A	-0.9813
121	E	A	-1.1089
122	A	A	0.0000
123	R	A	-1.6981
124	S	A	0.0000
125	L	A	0.0337
126	T	A	-0.4739
127	T	A	-0.8211
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6873
131	G	A	-1.4838
132	Y	A	-0.8407
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9255
136	N	A	-1.1432
137	Y	A	-0.1509
138	V	A	0.0000
139	A	A	0.3374
140	P	A	0.6753
141	V	A	1.7165