Project name: SH3_K108D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	KA108D
Energy difference between WT (input) and mutated protein (by FoldX)	3.1064 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1042
• Maximal score value: 1.8496
• Average score: -0.9397
• Total score value: -53.562

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5632
86	L	A	0.9214
87	F	A	1.0148
88	V	A	0.5158
89	A	A	0.0000
90	L	A	-0.1282
91	Y	A	-0.5670
92	D	A	-2.5541
93	Y	A	-1.9353
94	E	A	-2.6500
95	A	A	-2.6306
96	R	A	-2.9870
97	T	A	-2.6622
98	E	A	-3.1042
99	D	A	-3.0446
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2177
103	F	A	0.0000
104	H	A	-2.7011
105	K	A	-2.3656
106	G	A	-1.3803
107	E	A	-1.2125
108	D	A	-0.4696	mutated: KA108D
109	F	A	0.0000
110	Q	A	-0.4477
111	I	A	-0.0223
112	L	A	0.1421
113	N	A	-0.8881
114	S	A	-1.1790
115	S	A	-1.5957
116	E	A	-2.5591
117	G	A	-2.1323
118	D	A	-2.4437
119	W	A	-1.1023
120	W	A	-1.0557
121	E	A	-1.1533
122	A	A	0.0000
123	R	A	-1.6834
124	S	A	0.0000
125	L	A	0.1270
126	T	A	-0.4302
127	T	A	-0.8021
128	G	A	-1.3540
129	E	A	-2.2395
130	T	A	-1.6928
131	G	A	-1.5008
132	Y	A	-0.8689
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9205
136	N	A	-1.1505
137	Y	A	-0.1265
138	V	A	0.0000
139	A	A	0.4460
140	P	A	0.8165
141	V	A	1.8496