Project name: SH3_T130G

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	TA130G
Energy difference between WT (input) and mutated protein (by FoldX)	1.35315 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1122
• Maximal score value: 1.7964
• Average score: -1.0123
• Total score value: -57.6985

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.4768
86	L	A	0.6886
87	F	A	0.8773
88	V	A	0.4078
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6262
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1033
99	D	A	-3.1122
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2507
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3042
108	K	A	-0.7307
109	F	A	0.0000
110	Q	A	-0.7977
111	I	A	-0.1817
112	L	A	0.2087
113	N	A	-0.8140
114	S	A	-1.1410
115	S	A	-1.5753
116	E	A	-2.5736
117	G	A	-2.1443
118	D	A	-2.4557
119	W	A	-1.1267
120	W	A	-1.1078
121	E	A	-1.3570
122	A	A	0.0000
123	R	A	-2.2370
124	S	A	0.0000
125	L	A	-0.0573
126	T	A	-0.4758
127	T	A	-0.8607
128	G	A	-1.4500
129	E	A	-2.4912
130	G	A	-2.2482	mutated: TA130G
131	G	A	-1.7773
132	Y	A	-1.0175
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9330
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7556
141	V	A	1.7964