Project name: SH3_E121S

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	EA121S
Energy difference between WT (input) and mutated protein (by FoldX)	1.17234 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.0956
• Maximal score value: 1.7964
• Average score: -0.9143
• Total score value: -52.1161

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5183
86	L	A	0.7945
87	F	A	0.9250
88	V	A	0.4313
89	A	A	0.0000
90	L	A	-0.1511
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6181
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.0956
99	D	A	-2.9709
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.1988
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4581
107	E	A	-1.3078
108	K	A	-0.6420
109	F	A	0.0000
110	Q	A	-0.4158
111	I	A	0.1240
112	L	A	0.4100
113	N	A	-0.7971
114	S	A	-1.1084
115	S	A	-1.5740
116	E	A	-2.5371
117	G	A	-2.1091
118	D	A	-2.4016
119	W	A	-0.9943
120	W	A	-0.8128
121	S	A	-0.6440	mutated: EA121S
122	A	A	0.0000
123	R	A	-1.5704
124	S	A	0.0000
125	L	A	0.0585
126	T	A	-0.4730
127	T	A	-0.8214
128	G	A	-1.3587
129	E	A	-2.2431
130	T	A	-1.5521
131	G	A	-1.2888
132	Y	A	-0.6053
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8506
136	N	A	-1.1500
137	Y	A	-0.1241
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964