Project name: SH3_W119R

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA119R
Energy difference between WT (input) and mutated protein (by FoldX)	1.15055 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.5951
• Maximal score value: 1.7964
• Average score: -1.1636
• Total score value: -66.3274

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9232
88	V	A	0.4303
89	A	A	0.0000
90	L	A	-0.1542
91	Y	A	-0.5799
92	D	A	-2.5690
93	Y	A	-1.9537
94	E	A	-2.6539
95	A	A	-2.6385
96	R	A	-2.9873
97	T	A	-2.6610
98	E	A	-3.1071
99	D	A	-3.0526
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2165
103	F	A	0.0000
104	H	A	-2.7333
105	K	A	-2.4098
106	G	A	-1.4592
107	E	A	-1.3102
108	K	A	-0.6438
109	F	A	0.0000
110	Q	A	-0.5091
111	I	A	-0.0605
112	L	A	0.1393
113	N	A	-1.2239
114	S	A	-1.6765
115	S	A	-1.8976
116	E	A	-3.0108
117	G	A	-2.8207
118	D	A	-3.5951
119	R	A	-3.4526	mutated: WA119R
120	W	A	-2.2369
121	E	A	-1.7179
122	A	A	0.0000
123	R	A	-1.7201
124	S	A	0.0000
125	L	A	0.0587
126	T	A	-0.4736
127	T	A	-0.8209
128	G	A	-1.3572
129	E	A	-2.2402
130	T	A	-1.6997
131	G	A	-1.7926
132	Y	A	-1.6103
133	I	A	0.0000
134	P	A	-1.5357
135	S	A	-1.6959
136	N	A	-1.5118
137	Y	A	-0.3873
138	V	A	0.0000
139	A	A	0.4154
140	P	A	0.7757
141	V	A	1.7964