Project name: SH3_W120N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	WA120N
Energy difference between WT (input) and mutated protein (by FoldX)	3.15689 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.8431
• Average score: -0.9477
• Total score value: -54.0196

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5552
86	L	A	0.8664
87	F	A	1.0613
88	V	A	0.4984
89	A	A	0.0000
90	L	A	-0.1344
91	Y	A	-0.5743
92	D	A	-2.5596
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4057
106	G	A	-1.4418
107	E	A	-1.2872
108	K	A	-0.5967
109	F	A	0.0000
110	Q	A	-0.4778
111	I	A	-0.0575
112	L	A	0.1073
113	N	A	-0.9712
114	S	A	-1.3212
115	S	A	-1.6680
116	E	A	-2.6249
117	G	A	-2.2101
118	D	A	-2.5269
119	W	A	-1.2187
120	N	A	0.0000	mutated: WA120N
121	E	A	-1.2520
122	A	A	0.0000
123	R	A	-1.7261
124	S	A	0.0000
125	L	A	0.0733
126	T	A	-0.4730
127	T	A	-0.8207
128	G	A	-1.3572
129	E	A	-2.2401
130	T	A	-1.6891
131	G	A	-1.5247
132	Y	A	-0.9336
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9949
136	N	A	-1.1835
137	Y	A	-0.1173
138	V	A	0.0000
139	A	A	0.4575
140	P	A	0.8347
141	V	A	1.8431