Project name: SH3_I111F

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	IA111F
Energy difference between WT (input) and mutated protein (by FoldX)	3.81304 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7871
• Average score: -0.9144
• Total score value: -52.1224

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.6133
86	L	A	0.9053
87	F	A	1.0101
88	V	A	0.4122
89	A	A	0.0000
90	L	A	-0.1543
91	Y	A	-0.5748
92	D	A	-2.5599
93	Y	A	-1.9315
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7282
105	K	A	-2.4059
106	G	A	-1.4614
107	E	A	-1.3179
108	K	A	-0.5991
109	F	A	0.0000
110	Q	A	-0.2358
111	F	A	0.5653	mutated: IA111F
112	L	A	0.4633
113	N	A	-0.6843
114	S	A	-0.9943
115	S	A	-1.5726
116	E	A	-2.5461
117	G	A	-2.1222
118	D	A	-2.4343
119	W	A	-1.0931
120	W	A	-0.8979
121	E	A	-0.9777
122	A	A	0.0000
123	R	A	-1.5868
124	S	A	0.0000
125	L	A	0.0211
126	T	A	-0.4881
127	T	A	-0.8294
128	G	A	-1.3688
129	E	A	-2.2470
130	T	A	-1.6285
131	G	A	-1.4375
132	Y	A	-0.7942
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.8496
136	N	A	-1.1504
137	Y	A	-0.1275
138	V	A	0.0000
139	A	A	0.4065
140	P	A	0.7617
141	V	A	1.7871