Project name: SH3_G106N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA106N
Energy difference between WT (input) and mutated protein (by FoldX)	3.05539 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 1.7069
• Average score: -0.9832
• Total score value: -56.0444

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5138
86	L	A	0.7879
87	F	A	0.8125
88	V	A	0.2378
89	A	A	0.0000
90	L	A	-0.2080
91	Y	A	-0.5358
92	D	A	-2.5053
93	Y	A	-1.8528
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.7217
105	K	A	-2.3522
106	N	A	-2.0490	mutated: GA106N
107	E	A	-1.5607
108	K	A	-0.7914
109	F	A	0.0000
110	Q	A	-0.5106
111	I	A	-0.0560
112	L	A	0.1429
113	N	A	-0.8879
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0557
121	E	A	-1.1530
122	A	A	0.0000
123	R	A	-1.7128
124	S	A	0.0000
125	L	A	0.0641
126	T	A	-0.5515
127	T	A	-0.8180
128	G	A	-1.3554
129	E	A	-2.2392
130	T	A	-1.6920
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1508
137	Y	A	-0.0695
138	V	A	0.0000
139	A	A	0.3244
140	P	A	0.7676
141	V	A	1.7069