Project name: SH3_V141N

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA141N
Energy difference between WT (input) and mutated protein (by FoldX)	1.28456 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 0.1378
• Average score: -1.1185
• Total score value: -63.7519

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	-0.1598
86	L	A	-0.1675
87	F	A	-0.1430
88	V	A	-0.4540
89	A	A	0.0000
90	L	A	-0.1090
91	Y	A	-0.5306
92	D	A	-2.5183
93	Y	A	-1.9312
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.6761
105	K	A	-2.2997
106	G	A	-1.5415
107	E	A	-1.2242
108	K	A	-1.1687
109	F	A	0.0000
110	Q	A	-0.5297
111	I	A	-0.0725
112	L	A	0.1378
113	N	A	-0.8903
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0578
121	E	A	-1.1558
122	A	A	0.0000
123	R	A	-1.7083
124	S	A	0.0000
125	L	A	0.1223
126	T	A	-0.4148
127	T	A	-0.8005
128	G	A	-1.3281
129	E	A	-2.2235
130	T	A	-1.6838
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1501
137	Y	A	-0.1346
138	V	A	0.0000
139	A	A	-0.3741
140	P	A	-0.7399
141	N	A	-1.2516	mutated: VA141N