Project name: SH3_G131D

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	GA131D
Energy difference between WT (input) and mutated protein (by FoldX)	7.78602 kcal/mol CAUTION: Your mutation/s can destabilize the protein structure Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.178
• Maximal score value: 1.7964
• Average score: -0.9893
• Total score value: -56.3919

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.5173
86	L	A	0.7932
87	F	A	0.9247
88	V	A	0.4331
89	A	A	0.0000
90	L	A	-0.1503
91	Y	A	-0.5741
92	D	A	-2.5589
93	Y	A	-1.9311
94	E	A	-2.6445
95	A	A	-2.6733
96	R	A	-2.9843
97	T	A	-2.6592
98	E	A	-3.1780
99	D	A	-3.1728
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2651
103	F	A	0.0000
104	H	A	-2.7262
105	K	A	-2.4041
106	G	A	-1.4553
107	E	A	-1.3021
108	K	A	-0.6399
109	F	A	0.0000
110	Q	A	-0.5070
111	I	A	-0.0982
112	L	A	0.0960
113	N	A	-0.8845
114	S	A	-1.1766
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4413
119	W	A	-1.1496
120	W	A	-1.1240
121	E	A	-1.2902
122	A	A	0.0000
123	R	A	-1.8089
124	S	A	0.0000
125	L	A	0.0623
126	T	A	-0.4716
127	T	A	-0.8176
128	G	A	-1.3998
129	E	A	-2.3364
130	T	A	-1.8946
131	D	A	-1.9390	mutated: GA131D
132	Y	A	-1.0662
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9169
136	N	A	-1.1504
137	Y	A	-0.1243
138	V	A	0.0000
139	A	A	0.4158
140	P	A	0.7757
141	V	A	1.7964