Project name: SH3_V141T

Chain sequence(s)	A: TLFVALYDYEARTEDDLSFHKGEEKFQQILNSSEGDWWEARRSLTTGETGYIPSNYVAPV
Distance of aggregation	10 Å
Dynamic mode	No
Mutated residues	VA141T
Energy difference between WT (input) and mutated protein (by FoldX)	0.813622 kcal/mol Changes in protein stability upon mutation are calculated using the FoldX forcefield. Computational prediction of protein stability is used with the intention of preventing the experimental characterization of proteins bearing mutations that significantly destabilize their structure. Mutations resulting in a predicted reduction in protein stability ≥ 1 kcal/mol are considered disruptive.

• Minimal score value: -3.1028
• Maximal score value: 0.3219
• Average score: -1.0426
• Total score value: -59.429

The table below lists A3D score for protein residues. Residues with A3D score > 0.0000 are marked by yellow rows.

residue index	residue name	chain	Aggrescan3D score	mutation
85	T	A	0.1408
86	L	A	0.2955
87	F	A	0.3219
88	V	A	-0.0823
89	A	A	0.0000
90	L	A	-0.1157
91	Y	A	-0.5392
92	D	A	-2.5221
93	Y	A	-1.9321
94	E	A	-2.6452
95	A	A	-2.6259
96	R	A	-2.9852
97	T	A	-2.6608
98	E	A	-3.1028
99	D	A	-3.0434
100	D	A	0.0000
101	L	A	0.0000
102	S	A	-2.2059
103	F	A	0.0000
104	H	A	-2.6731
105	K	A	-2.3012
106	G	A	-1.4211
107	E	A	-1.1982
108	K	A	-0.8814
109	F	A	0.0000
110	Q	A	-0.4882
111	I	A	-0.0476
112	L	A	0.1378
113	N	A	-0.8903
114	S	A	-1.1794
115	S	A	-1.5962
116	E	A	-2.5599
117	G	A	-2.1340
118	D	A	-2.4444
119	W	A	-1.1028
120	W	A	-1.0578
121	E	A	-1.1558
122	A	A	0.0000
123	R	A	-1.6885
124	S	A	0.0000
125	L	A	0.1561
126	T	A	-0.3981
127	T	A	-0.7921
128	G	A	-1.3281
129	E	A	-2.2235
130	T	A	-1.6838
131	G	A	-1.4992
132	Y	A	-0.8673
133	I	A	0.0000
134	P	A	0.0000
135	S	A	-0.9206
136	N	A	-1.1511
137	Y	A	-0.1396
138	V	A	0.0000
139	A	A	-0.0519
140	P	A	-0.1256
141	T	A	-0.0197	mutated: VA141T